Objective: To establish an HPLC fingerprint of different size of yacon leaves

and determine the content of chlorogenic acid and enhydrin. Method: The fingerprints were obtained on a Kromasil C18 (4.6 mm×250 mm

5 μm) with the gradient elution solvent system composed of acetonitrile and 0.4% phosphoric acid. The flow rate was 1.0 mL · min-1. The column temperature was maintained at 30 ℃

and the detection wavelength was 0-18 min

327 nm

18.1-60 min

210 nm. Result: The fingerprint of yacon leaves was established. Larger leaves samples with high content of chlorogenic acid(1.588 2 mg · g-1)

and the small yacon leaves with high content of enhydrin(5.637 4 mg · g-1). Conclusion: The chlorogenic acid and enhydrin content in yacon leaves was correlated with the size. This method is easy to operate with the high exactitude and the excellent repetition for the quantitative analysis of yacon leaves.