Objective: To investigate the effect of Tongxinluo capsule on adhesion molecule in diabetic peripheral neuropathy KK/Upj-Ay mice and its mechanisms. Method: KK/Upj-Ay mice were randomly divided into model

Tongxinluo low-

middle- and high group(4

2

1 g · kg-1). C57BL/6 mice were selected as control group. Mice were given intragastrically for 12 weeks. Motor nerve conduction velocity (MNCV) and sensory nerve conduction velocity (SNCV) were detected. The content of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1(VCAM-1) in blood were detected by flow cytometer(FCM). The expression of ICAM-1

VCAM-1 and nuclear factor kappa B (NF-κB) in sciatic nerve were examined by real time PCR and Western blot. Result: Compared with control group

MNCV and SNCV were slower

ICAM-1 and VCAM-1 contents in blood were increased and ICAM-1

VCAM-1 and NF-κB expression in sciatic nerve increased in model group(P<0.01). Compared with model group

MNCV and SNCV were faster and ICAM-1 and VCAM-1 contents were decreased in Tongxinluo middle

high group (P<0.05

P<0.01).The expression of ICAM-1

VCAM-1 and NF-κB mRNA and protein in sciatic nerve of Tongxinluo group significantly decreased (P<0.05

P<0.01). Conclusion: Tongxinluo capsule ameliorates diabetic peripheral neuropathy via inhibiting adhesion molecule expression in the diabetic peripheral neuropathy mice

and this is due to down-regulating NF-κB.