Objective: To observe the effects of tetramethylpyrazine(TMP) on the proliferation

migration

tube formation and secretion of the stromal cell-derived factor-1(SDF-1) and vascular endothelial cell growth factor(VEGF) from brain microvascular endothelial cells(BMECs). Method: Primary isolated BMECs of passage 3 to 6 were used in this experiment. BMECs that treated with TMP(2

10

50 mg · L-1) were set as TMP 2

TMP 10 and TMP 50 groups

and that treated with the same volume of medium was regarded as control. Effects of TMP on the proliferation of BMECs were detected by MTS and BrdU incorporation. Impact of TMP on migration of BMECs was observed by Transwell assay. The influence of TMP on tube formation of BMECs induced by VEGF was observed by tube formation in vitro. The level of SDF-1 and VEGF in BMECs culture supernatant were examined by ELISA. Result: BrdU incorporation and MTS results showed that TMP(2

10

50 mg · L-1) can significantly increase the proliferation of BMECs(P<0.05

P<0.01). TMP can dose-dependently promote BMECs migrate to the lower chamber of Transwell and increase the number of branch points in tube formation. In addition

TMP(2

10

50 mg · L-1) can significantly increase the level of SDF-1 and VEGF in cell culture supernatant (P<0.05

P<0.01). Conclusion: TMP could effectively promote BMECs proliferation

migration and tube formation. Meanwhile

it could increase the secretion of SDF-1 and VEGF from BMECs

which may be part of the mechanism for promoting angiogenesis.