Objective: To investigate the influence of Buyang Huanwu Tang (BHT)on the expressions of protein kinase B(AKT)and phosphorylated protein kinase B(p-AKT)in acute cerebral ischemia reperfusion rat. Method: The specific pathogen free SD rats were randomly divided into sham operation group

model group

positive control group and BHT(high

low dose) groups

each group contains 20 rats

Clopidogrel dose was 6.8 mg · kg-1

and BHT high low dose was26

6.5 g · kg-1. The other groups were given distilled water. The acute cerebral ischemia-reperfusion models were established by bilateral carotid artery occlusion

using HE staining to observe Bcerebral pathological histology

using immunohistochemical method to detect brain cortex and hippocampus CA1 area AKT and p-AKT positive expression level. Result: BHT could significantly improve the degree of acute cerebral ischemia-reperfusion injury in rat brain tissue.HE Staining showed that the number of cortex neuron cells of acute cerebral ischemia-reperfusion rats decreased

cell arranged loosely and not regular

cell nucleus become atrophy

cells surrounding space became big obviously. The number of hippocampus CA1 area neurons decreased significantly

disordered arrangement of cells

and each dose group tissue damage degree were eased. Immunohistochemical results showed that each group of cortex and hippocampus CA1 area were seen AKT(Ab473)and p-AKT (ser473) positive expression. Compared with the sham operation group

model group AKT (Ab473) positive expression had no significant difference. Compared with model group

positive control group

BHT high dose group positive expression of AKT was obviously increased (P<0.05). Conclusion: The neuroprotective effect of Buyang Huanwu Tang against acute cerebral ischemia-reperfusion in rat may be associated with upregulation of AKT and p-AKT.