Objective: To Compare metabolism and tissue distribution in osteoporosis model rats treated by single icariin and compound containing icariin. Method: The rat models of osteoporosis was established by ovariectomy in female SPF rats.Tissues were collected after oral administration of 118 mg · kg-1 icariin 40 min

1.5

4

24 h. The concentration of each component was determined by HPLC after sample preparation. The column was Agilent Eclipse XDB-C18 (4.6 mm×250 mm

5 μm) and column temperature was 35 ℃;Mobile phase was A(methanol)-B(0.2%H3PO4 in water);the velocity of flow was 1.0 mL · min-1;the detection wavelength was 270 nm. Result: Icariinis widely distributed in rats

high concentration distributed in the kidney

liver

spleen

muscle tissue

and its metabolites coexisted in the organization. Comparison of compound Bushen Zhuanggu and icariinis monomer in the same time and the same organization was executed. In muscle:icariin

icariside Ⅱ

icaritin had a high concentration and slow elimination

icaritin at 4

24 h and icariside Ⅱat 4 h the significant difference was found(P<0.05). In kidney:icariin at 40 min

4

24 h and icariside Ⅰ

icaritin at 24 h was detected

and had a significant difference between groups(P<0.05). In liver:Icariin at 4

24 h

icaritin at 4

24 h was detected

the significant difference between groups was found(P<0.05). Conclusion: The distribution of icariin in compound prescription had slower absorption

distribution

longer action time

slower elimination than in single

which may be related to interaction between the chemical composition of compounds.