Objective: To explore the immuno-neurologic regulation of renal hypertensive and its inherent law

and to investigate the mechanisms of curing and systemic regulating effect of Ziyin Qianyang formula. Method: Renal hypertension rats (RHR) model was established by two kidney one clip (2K1C) and 2K1C-RHR were divided into six groups:the model group

the high-

medium-

low- dose Zinyin Qianyang formula groups(32

16

8 g · kg-1)

the losartan potassium group (0.005 g · kg-1) and the Tianma Gouteng particles group (1.5 g · kg-1). Drugs were given by intragastric administration for 4 weeks. The blood pressure was measured by a non-invasive blood pressure monitor. The contents of plasma angiotensin Ⅱ (AngⅡ) were detected by radioimmunity method. The protein levels of angiotensin Ⅱ type l receptor (AT1R) and rabbit-anti protein kinase C (p-PKC) were detected by Western blotting. The contents of inositol triphosphate(IP3) and diacylglycerol(DAG) in peripheral blood lymphocytes and the levels of interlenkin-4(IL-4)

IL-6 in the blood serum of RHR were detected using ELISA. Result: Compared with the model group

Ziyin Qianyang formula could decrease blood pressure of RHR significantly (P<0.01). The medium-

low-dose Ziyin Qianyang formula could decrease the concentration of plasma AngⅡ (P<0.05). The high-

medium-dose Ziyin Qianyang formula could decrease the concentration of AT1R and p-PKC in peripheral blood lymphocytes of rats (P<0.05

P<0.01). The high-dose Ziyin Qianyang formula could decrease the concentration of IP3 and DAG (P<0.05

P<0.01) in peripheral blood lymphocytes and the levels of IL-4

IL-6 in blood serum of RHR (P<0.05

P<0.01). Conclusion: The renal hypertensive may be related to the excessive expression of AT1R in peripheral blood lymphocytes and the activation of IP3/DAG signaling pathway

where Ziyin Qianyang formula could exert immuno-neurologic regulation action.