Objective: To study the serum containing drug of different processed products of Qing'e Pill on osteoblast proliferation

differentiation and mineralization. This study was designed to evaluate the promoting effect of serum containing drug of different processed products of Qing'e Pill on human osteoblast and investigate the possible mechanism in the treatment of osteoporosis. Method: Osteogenic cells were obtained from the femur in postmenopausal women. Different processed products of Qing'e Pill were performed by using crude and salt-roast Eucommia ulmoides and Psoralea corylifolia

respectively. Female Sprague Dawley rats were orally administrated with water extract of Qing'e Pill for 7 days. At the end of the administration

serum containing drug was collected. The experiment was divided into the blank group

the blank serum group

the different processed products of Qing'e Pill groups. Cell proliferation was determined by MTT assay. Cell (activity of alkaline phosphatase) ALP activity and mineralized nodule were tested using ELISA. Result: Compared to the blank serum group

the different processed products of Qing'e pill could significantly promote huaman osteoblast proliferation and ALP activity (P<0.05). Moreover

the formation of bone nodules increased in the osteoblasts after 14-day treatment in different processed products of Qing'e pill groups (P<0.05). Meanwhile

the ALP (P<0.01) and mineralization activity results of the salt-roast were better than the crude. Conclusion: Qing'e pill could increase proliferation

differentiation and mineralization activities in human osteoblast. Its therapeutic effect of osteoporosis might be involved in increasing the osteogenic function. Comparing to the crude drug

the salt-roast has a better effect in treating osteoporosis.