Objective: To optimize extraction technology of Tianma Anshen granules. Method: With dry extract rate and contents of gastrodin

schizandrin

rhynchophylline as comprehensive index

orthogonal design was adopted to investigate effects of the amount and concentration of ethanol

extracting time and times on extraction technology.Contents of gastrodin

schizandrin and rhynchophylline were determined by HPLC

mobile phase of gastrodin was acetonitrile-0.05% phosphoric acid (3:97)

detection wavelength was 220 nm;mobile phase of schizandrin and rhynchophylline was acetonitrile-0.001% triethylamine (45:55)

detection wavelength was 250 nm. Result: Optimal extraction technology was extracted 3 times with 6 times the amount of 50% ethanol for 1 h per time.Extraction amounts of gastrodin

schizandrin and rhynchophylline were 2.69

1.47

0.18 mg·g-1

dry extract rate was 22.64%. Conclusion: This optimized extraction technology is stable

feasible and suitable for industrial production of Tianma Anshen granules.