Objective: To investigate the chromatographic fingerprints of volatile oil from Epimedii Folium by GC-MS. Method: The volatile oil was extracted from Epimedii Folium by steam distillation method

chromatographic fingerprints of which was detected and calculated by GC-FID

and the mutual fingerprints of ten kinds essential oil from Epimedii Folium was established. GC conditions:HP-INNOWAX column inlet temperature 260 ℃

the split ratio of 5:1;temperature program:initial temperature of 60 ℃

keep 10 min

with 2 ℃·min-1 rose to 250 ℃

13 min to maintain. GC-MS parameters:column flow rate 1.0 mL·min-1

MS electron energy 70 eV

ion source temperature of 230 ℃

the quadrupole temperature 180 ℃

the transmission line temperature 280 ℃

full-ion scan mass range m/z 40-550

solvent delay 3 min. GC-FID parameters:column flow rate 1.5 mL·min-1

FID detector temperature 260 ℃

the hydrogen flow rate 30 mL·min-1

the air flow 300 mL·min-1

tail blowing nitrogen 30 mL·min-1. Result: The method was proved to be accuracy

reproducible and stability

by which the ideal fingerprints of volatile oil from Epimedii Folium were obtained. Conclusion: The methods set up are beneficial for the quality control of Epimedii Folium.