假益智果实挥发油成分分析及其抑菌活性

肖文琳; 宋小平; 陈光英; 陈文豪; 刘鹤; 高歌; 韩长日

doi:10.13422/j.cnki.syfjx.2015070047

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假益智果实挥发油成分分析及其抑菌活性

化学与分析 | 更新时间：2024-09-23

- 假益智果实挥发油成分分析及其抑菌活性
- Chemical Composition and Antibacterial Activity of Volatile Oil from Fruits of
- 中国实验方剂学杂志 2015年21卷第7期页码：47-50
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目(81160391,21362009,81360478);国家国际科技合作专项(2014DFA40850)
- DOI：10.13422/j.cnki.syfjx.2015070047
  中图分类号： R284.1
- 纸质出版日期：2015
- 稿件说明：
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肖文琳, 宋小平, 陈光英, 等. 假益智果实挥发油成分分析及其抑菌活性[J]. 中国实验方剂学杂志, 2015,21(7):47-50.

XIAO Wen-lin, SONG Xiao-ping, CHEN Guang-ying, et al. Chemical Composition and Antibacterial Activity of Volatile Oil from Fruits of [J]. Chinese journal of experimental traditional medical formulae, 2015, 21(7): 47-50.
肖文琳, 宋小平, 陈光英, 等. 假益智果实挥发油成分分析及其抑菌活性[J]. 中国实验方剂学杂志, 2015,21(7):47-50. DOI： 10.13422/j.cnki.syfjx.2015070047.

XIAO Wen-lin, SONG Xiao-ping, CHEN Guang-ying, et al. Chemical Composition and Antibacterial Activity of Volatile Oil from Fruits of [J]. Chinese journal of experimental traditional medical formulae, 2015, 21(7): 47-50. DOI： 10.13422/j.cnki.syfjx.2015070047.

摘要

目的: 对假益智果实挥发油进行化学成分研究

并进行抗菌活性测试

为进一步研究开发假益智提供实验依据。方法: 使用水蒸气蒸馏法从假益智的果实中提取出挥发油

运用气相色谱-质谱(GC-MS)联用技术进行分析;使用微量稀释法测试挥发油的抗菌活性。结果: 假益智果实挥发油提取率为0.17%

检测到42个化合物

鉴定出31个

占挥发油总成分的94.18%。挥发油对四联球菌具有很好的抑菌作用

最小抑制浓度为12.5 g·L-1

对白色葡萄球菌、蜡状芽孢杆菌、金黄色葡萄球菌、枯草芽孢杆菌、大肠埃希菌细菌具有中等强度的抑制作用。结论: 本文对假益智果实进行挥发油提取鉴定

其主要成分为萜类、长链烃类化合物。抗菌实验结果表明假益智果实挥发油对四联球菌具有较好的抗菌活性。

Abstract

Objective: To study the chemical constituents and antibacterial activity of volatile oil from the fruits of Alpinia maclurei. Method: The volatile oils were extracted from fruits of A. maclurei by steam distillation

and its ingredients were identified by GC-MS. The antibacterial activity of volatile oils was tested using microdilution method. Result: 42 chromatographic peaks were detected and 31 compounds were identified

which constituted about 94.18% of the total volatile oil. The volatile oil of A. maclurei showed inhibitory activity against staphylococcus albus

bacillus cereus

staphylococcus aureus

bacillus subtilis

escherichia coli and exhibited higher activity to inhibit pediococcus aureus

with minimum inhibitory concentration (MIC) value of 12.5 g·L-1. Conclusion: Volatile oil was extracted from A. maclurei and the compounds were identified for the first time

containing many reactive components. The oil was found to possess antimicrobial activity against Pediococcus.

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