HPLC测定跌打伤药凝胶膏剂中血竭素和延胡索乙素的含量

李江英; 赵锋; 吕卓; 孙哲; 张亚军

doi:10.13422/j.cnki.syfjx.2015070057

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HPLC测定跌打伤药凝胶膏剂中血竭素和延胡索乙素的含量

化学与分析 | 更新时间：2024-09-23

- HPLC测定跌打伤药凝胶膏剂中血竭素和延胡索乙素的含量
- Determination of Dracohodin and Tetrahydropulmatine in Dieda Shangyao Cataplasm by HPLC
- 中国实验方剂学杂志 2015年21卷第7期页码：57-60
- 作者机构：
- 作者简介：
- 基金信息：
  
  陕西省科学研究发展计划项目(2013k12-07-06);西安市科技计划项目(sf1205)
- DOI：10.13422/j.cnki.syfjx.2015070057
  中图分类号： O657.72;R286.0
- 纸质出版日期：2015
- 稿件说明：
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李江英, 赵锋, 吕卓, 等. HPLC测定跌打伤药凝胶膏剂中血竭素和延胡索乙素的含量[J]. 中国实验方剂学杂志, 2015,21(7):57-60.

LI Jiang-ying, ZHAO Feng, LYU Zhuo, et al. Determination of Dracohodin and Tetrahydropulmatine in Dieda Shangyao Cataplasm by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(7): 57-60.
李江英, 赵锋, 吕卓, 等. HPLC测定跌打伤药凝胶膏剂中血竭素和延胡索乙素的含量[J]. 中国实验方剂学杂志, 2015,21(7):57-60. DOI： 10.13422/j.cnki.syfjx.2015070057.

LI Jiang-ying, ZHAO Feng, LYU Zhuo, et al. Determination of Dracohodin and Tetrahydropulmatine in Dieda Shangyao Cataplasm by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(7): 57-60. DOI： 10.13422/j.cnki.syfjx.2015070057.

摘要

目的: 建立同时测定跌打伤药凝胶膏剂中血竭素和延胡索乙素的方法。方法: 采用C18色谱柱 (4.6 mm ×250 mm

5 μm)

流动相甲醇-0.1%磷酸水溶液(三乙胺调pH至6.0) (60:40)

流速0.8 mL·min-1

程序可变检测波长 (0～20 min为440 nm

20～35 min为280 nm)

柱温30 ℃

进样量10 μL。结果: 血竭素和延胡索乙素分别在0.037 0～0.592 8

0.026 50～0.424 0 μg峰面积与进样量呈良好的线性关系

平均回收率分别为98.1%(RSD 1.4%)

96.7%(RSD 1.7%)。结论: 该方法简便、准确、重复性好

可用于跌打伤药凝胶膏剂的质量控制。

Abstract

Objective: To establish a method for simultaneous determination of dracohodin and tetrahydropulmatine in Dieda Shangyao cataplasm. Method: A high performance liquid chromatographic (HPLC) method was adopted with programmed wavelength UV detection. A C18 column(4.6 mm×250 mm

5 μm)was used for the analysis at 30 ℃. The separation was carried out with the mobile phase consisting of methanol and 0.1% phosphoric acid (60:40

adjusted to pH 6.0 with triethylamine) at a flow rate of 0.8 mL·min-1

and the sample size was 10 μL. The eluate was detected by programmed wavelength (0-20 min

440 nm;20-35 min

280 nm). Result: The linearities were from 0.037 0 to 0.592 8 μg for dracohodin

0.026 5 to 0.424 0 μg for tetrahydropulmatine. The average recoveries of dracohodin and tetrahydropulmatine were 98.1%(RSD 1.4%)and 96.7%(RSD 1.7%)

respectively. Conclusion: The established method was simple

accurate and highly reproducible that it could be used for quality control of Dieda Shangyao cataplasm.

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