Objective: To establish a method for determination of chlorogenic acid

galuteolin and dipsaeoside B in Lonicerae Flos using Ultra Performance Liquid Chromatography

and inspect the quality of the Lonicerae Flos in Guizhou. Method: ACQUITY UPLC BEH C18(2.1 mm× 100 mm

1.7 μm) column was adopted. Two solution were acetonitrile and water containing 0.4% acetic acid

with gradient elution of (0-2 min

7% A;2-2.3 min

7%-28% A;2.3-10 min

28% A;10-10.5 min

28%-7% A)

and velocity gradient(0-2 min

0.35 mL·min-1;2-2.3 min

0.35-0.25 mL·min-1;2.3-2.6 min

0.25-0.15 mL·min-1;2.6-2.8 min

0.15-0.1 mL·min-1;2.8-4 min

0.1-0.2 mL·min-1;4-4.3 min

0.2-0.3 mL·min-1;4.3-4.5 min

0.3-0.35 mL·min-1;4.5-10.5 min

0.35 mL·min-1). The PDA detection wavelength was 330 nm for chlorogenic aicd. The ELSD detection was for macranthoidin B and dipsacoside B. The temperature of drift tube was maintained at 60 ℃

the temperature of nebulizer was 48 ℃

with the nitrogen flow rate of 20 psi

and the column temperature was maintained at 50 ℃. Result: The linear response ranges of chlorogenic acid

galuteolin and dipsaeoside B were 0.304 5-1.522 5(R2=0.999 4)

0.449-1.436 8(R2=0.9993)

0.044 6-0.223 μg (R2=0.999 4)

respectively. Conclusion: The method is convenient and accurate to determine the content of chlorogenic acid

macranthoidin B and dipsacoside B in Lonicerae Flos

and can shorten the measuring time and saves reagent effectively. The established method has used to measure quality of the Lonicerae Flos in Guizhou.