Objective: To investigate and screen the antibacterial activity of different extracts from Paliurus ramosissimus folium. Method: The P. ramosissimus folium was extracted by systematic solvents method and separated into five parts:petroleum ether extract

chloroform extract

ethyl acetate extract

n-butyl alcohol extract and water extract. The in vitro antibacterial activity on four strains of these extracts was screened. These four strains were Escherichia coli

Bacillus subtilis

Staphylococcus aureus and Salmonella. The minimal inhibitory concentration (MIC) of the extracts was measured by the spread plate with double dilution drugs. Result: The extracts of petroleum ether

chloroform and water had no antibacterial activity. The extracts of ethyl acetate and n-butyl alcohol had different degree of antibacterial activity. n-butyl alcohol extract had obvious antibacterial activity with MIC of 0.714

0.714

0.357

0.357 g·L-1

respectively. Ethyl acetate extract had antibacterial activity on four strains. Their potency of antibacterial activity was as follows:n-butyl alcohol extract > ethyl acetate extract> water extract. Conclusion: The extracts of n-butyl alcohol and ethyl acetate were the inhibitory effective sites of P. ramosissimus folium. The results provide a basis for developing new bacteriostatic drug.