一阶导数UV测定独一味及不同提取物中苯乙醇苷含量

邱建国; 张泉龙; 李茂星; 贾正平; 张汝学; 赵一; 王谨涵; 陶锐

doi:10.13422/j.cnki.syfjx.2015080043

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一阶导数UV测定独一味及不同提取物中苯乙醇苷含量

化学与分析 | 更新时间：2024-01-04

- 一阶导数UV测定独一味及不同提取物中苯乙醇苷含量
- Determination of Phenylethanol Glycosides in Different Extract of Lamiophlomis Herba by First Derivative Ultraviolet Spectrophotometry
- 中国实验方剂学杂志 2015年21卷第8期页码：43-46
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家科技重大专项(2008ZXJ09004-XXX);甘肃省科技重大专项(1102FKDA012);甘肃省中医药管理局科研课题(GZK-2014-1);全军中医药研发推广项目(2006032001)
- DOI：10.13422/j.cnki.syfjx.2015080043
  中图分类号： R284;R285
- 纸质出版日期：2015
- 稿件说明：
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邱建国, 张泉龙, 李茂星, 等. 一阶导数UV测定独一味及不同提取物中苯乙醇苷含量[J]. 中国实验方剂学杂志, 2015,21(8):43-46.

QIU Jian-guo, ZHANG Quan-long, LI Mao-xing, et al. Determination of Phenylethanol Glycosides in Different Extract of Lamiophlomis Herba by First Derivative Ultraviolet Spectrophotometry[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(8): 43-46.
邱建国, 张泉龙, 李茂星, 等. 一阶导数UV测定独一味及不同提取物中苯乙醇苷含量[J]. 中国实验方剂学杂志, 2015,21(8):43-46. DOI： 10.13422/j.cnki.syfjx.2015080043.

QIU Jian-guo, ZHANG Quan-long, LI Mao-xing, et al. Determination of Phenylethanol Glycosides in Different Extract of Lamiophlomis Herba by First Derivative Ultraviolet Spectrophotometry[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(8): 43-46. DOI： 10.13422/j.cnki.syfjx.2015080043.

摘要

目的: 建立独一味水提物、水洗脱物和70%乙醇溶液洗脱物中总苯乙醇苷含量的测定方法。方法: 加独一味药材质量18倍量水

煎煮提取3次

每次1.5 h

滤过

合并滤液

滤液2～4 ℃冷藏除尘

60 ℃减压干燥得到水提物

水提物经聚酰胺和大孔树脂富集分离

分别用水和70%乙醇溶液洗脱

分别得到水洗脱物

70%乙醇溶液洗脱物Ⅰ(通过聚酰胺柱洗脱物)和70%乙醇溶液洗脱物Ⅱ(通过大孔树脂柱洗脱物);在224

252

256

354 nm波长处

采用一阶导数紫外分光光度法测定总苯乙醇苷的含量。结果: 建立的一阶导数紫外分光光度法测定总苯乙醇苷的含量

在354 nm波长处加样回收率99.71%

RSD 2.5%。结论: 该方法专属性强、灵敏度高、精确度与重复性好

适用于独一味中总苯乙醇苷的含量测定。

Abstract

Objective: To determine the total phenylethanol glycosides in water extract

water eluate and 70% ethanol eluate of Lamiophlomis Herba by first derivative ultraviolet spectrophotometry. Method: Lamiophlomis Herba was extracted with water (18 times) 3 times

1.5 h per time. The water extract was storage at 2-4 ℃

was dried under reduced pressure at 60 ℃. Water extract were separated and enriched by polyamide and macroporous resin

to obtain the water eluate and 70% ethanol eluate Ⅰ (polyamide column)

70% ethanol eluate Ⅱ (macroporous resin column). The total phenylethanol glycosides were determined by first derivative ultraviolet spectrophotometry at 224

252

256

354 nm. Result: Recovery of the total phenylethanol glycosides was 99.71%

RSD was 2.5% at 354 nm. Conclusion: This method is special

sensitive

accurate and repeatable

and can be used for the quality analysis of the total phenylethanol glycosides in Lamiophlomis Herba.

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