HPLC同时测定小柴胡汤乙酸乙酯部位中5种成分

原红霞; 王婷婷; 闫艳

doi:10.13422/j.cnki.syfjx.2015080064

您当前的位置：

首页 >

文章列表页 >

HPLC同时测定小柴胡汤乙酸乙酯部位中5种成分

化学与分析 | 更新时间：2024-01-04

- HPLC同时测定小柴胡汤乙酸乙酯部位中5种成分
- Simultaneous Determination of Five Components in Ethyl Acetate Extract of Xiao Chaihu Tang by HPLC
- 中国实验方剂学杂志 2015年21卷第8期页码：64-66
- 作者机构：
- 作者简介：
- 基金信息：
  
  山西省科技厅青年科技研究基金项目(2011021007-2)
- DOI：10.13422/j.cnki.syfjx.2015080064
  中图分类号： O657.72;R286.0
- 纸质出版日期：2015
- 稿件说明：
移动端阅览
原红霞, 王婷婷, 闫艳. HPLC同时测定小柴胡汤乙酸乙酯部位中5种成分[J]. 中国实验方剂学杂志, 2015,21(8):64-66.

YUAN Hong-xia, WANG Ting-ting, YAN Yan. Simultaneous Determination of Five Components in Ethyl Acetate Extract of Xiao Chaihu Tang by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(8): 64-66.
原红霞, 王婷婷, 闫艳. HPLC同时测定小柴胡汤乙酸乙酯部位中5种成分[J]. 中国实验方剂学杂志, 2015,21(8):64-66. DOI： 10.13422/j.cnki.syfjx.2015080064.

YUAN Hong-xia, WANG Ting-ting, YAN Yan. Simultaneous Determination of Five Components in Ethyl Acetate Extract of Xiao Chaihu Tang by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(8): 64-66. DOI： 10.13422/j.cnki.syfjx.2015080064.

摘要

目的: 建立同时测定小柴胡汤乙酸乙酯部位中甘草苷、黄芩苷、汉黄芩苷、黄芩素和汉黄芩素含量的方法。方法: 采用Diamonsil C18(2)色谱柱 (4.6 mm×250 mm

5.0 μm)

以乙腈-0.1%磷酸水溶液为流动相

梯度洗脱

流速1 mL·min-1

检测波长276 nm

柱温30 ℃。结果: 甘草苷、黄芩苷、汉黄芩苷、黄芩素和汉黄芩素的线性范围分别为0.162～1.62

0.24～2.4

0.206～2.06

0.221～2.21

0.077～0.77 μg

平均加样回收率分别为98.2%

103.3%

97.9%

100.6%

103.0%。结论: 该方法简便

快速

重复性好

可用于同时测定小柴胡汤乙酸乙酯部位中5种有效成分的含量。

Abstract

Objective: To establish a method for simultaneously determining liguiritin

wogonoside

baicalein and wogonin in ethyl acetate extract of Xiaochaihu Tang by HPLC. Method: The separation was performed on Diamonsil C18(2) (4.6 mm×250 mm

5 μm). The mobile phase consisted of acetonitrile and 0.1% phosphate acid was used as gradient elution

flow rate 1 mL·min-1.The detection wavelength was set at 276 nm. The column temperature was at 30 ℃. Result: Quantitative analysis of HPLC showed that the linear ranges of liquiritin

baicalin

wogonoside

baicalein and wogonin were 0.162-1.62

0.24-2.4

0.206-2.06

0.221-2.21

0.077-0.77 μg respectively. The average recovery was 98.2%

103.3%

97.9%

100.6%

103.0%. Conclusion: The method is simple

fast

reliable

and suitable for the quality control of ethyl acetate extract of Xiao Chaihu Tang.

关键词

Keywords

references

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

UPLC同时测定银黄颗粒中4种黄酮类成分含量

葛根芩连汤中黄芩活性成分在大鼠体内的组织分布

星点设计-效应面法优化黄芩中4种黄酮类成分的提取工艺

UPLC测定银黄颗粒中绿原酸、咖啡酸、黄芩苷、汉黄芩苷、黄芩素和汉黄芩素6种成分

HPLC同时测定黄栀花口服液中绿原酸、栀子苷、黄芩苷、黄芩素和汉黄芩素的含量