LIU Yu-hong, ZHANG Wen-yu, JIANG Hai-qiang. Chemical Constituents from the Fruits of and Their Neurotrophic Activity[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(8): 67-69.
LIU Yu-hong, ZHANG Wen-yu, JIANG Hai-qiang. Chemical Constituents from the Fruits of and Their Neurotrophic Activity[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(8): 67-69. DOI: 10.13422/j.cnki.syfjx.2015080067.
Objective: To isolate neurotrophic activity compounds from the fruits of Leonurus japonicus. Method: Silica gel
Sephadex LH-20 and preparative HPLC were used for isolation and purification. The structures of compounds were elucidated by MS and NMR spectra. Neurotrophic activity was evaluated on the neurite outgrowth of PC12 cells. Result: Five compounds were obtained and identified as aurantiamide acetate (1)
auraptenol (2)
1-monolinolein (3)
acacetin (4)
and β-sitosterol glucopyranoside (5). Compound 1 could significantly enhance the neurite outgrowth of PC12 cells. Conclusion: Compounds 1-5 were isolated for the first time from the genus Leonurus