HPLC同时测定甘蒲祛斑凝胶中甘草素、异甘草素、甘草查尔酮A的含量

左婷; 翁立冬; 刘莉; 张璐; 乡世健; 洪军辉; 刘强; 朱红霞

doi:10.13422/j.cnki.syfjx.2015080073

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HPLC同时测定甘蒲祛斑凝胶中甘草素、异甘草素、甘草查尔酮A的含量

化学与分析 | 更新时间：2024-01-04

- HPLC同时测定甘蒲祛斑凝胶中甘草素、异甘草素、甘草查尔酮A的含量
- Simultaneous Determination of Liquiritigenin, Isoliquiritigenin and Licochalcone A in Ganpuquban Gel by HPLC
- 中国实验方剂学杂志 2015年21卷第8期页码：73-76
- 作者机构：
- 作者简介：
- 基金信息：
  
  广州市白云区科技计划重点项目(2011-KQ-23);广东省科技计划重点项目(2011A030100006);广东省高校中药化妆品工程中心建设项目(GCZX-A1007);广州市白云区科技计划项目(2012-KZ-28)
- DOI：10.13422/j.cnki.syfjx.2015080073
  中图分类号： O657.72;R286.0
- 纸质出版日期：2015
- 稿件说明：
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左婷, 翁立冬, 刘莉, 等. HPLC同时测定甘蒲祛斑凝胶中甘草素、异甘草素、甘草查尔酮A的含量[J]. 中国实验方剂学杂志, 2015,21(8):73-76.

ZUO Ting, WENG Li-dong, LIU Li, et al. Simultaneous Determination of Liquiritigenin, Isoliquiritigenin and Licochalcone A in Ganpuquban Gel by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(8): 73-76.
左婷, 翁立冬, 刘莉, 等. HPLC同时测定甘蒲祛斑凝胶中甘草素、异甘草素、甘草查尔酮A的含量[J]. 中国实验方剂学杂志, 2015,21(8):73-76. DOI： 10.13422/j.cnki.syfjx.2015080073.

ZUO Ting, WENG Li-dong, LIU Li, et al. Simultaneous Determination of Liquiritigenin, Isoliquiritigenin and Licochalcone A in Ganpuquban Gel by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(8): 73-76. DOI： 10.13422/j.cnki.syfjx.2015080073.

摘要

目的: 建立同时测定甘蒲祛斑凝胶中甘草素、异甘草素、甘草查尔酮A含量的方法。方法: 采用高效液相色谱法

Agilent HC-C18色谱柱 (4.6 mm×250 mm

5 μm)

流动相0.05%磷酸水(A)-乙腈(B)梯度洗脱(0~4 min

80%A;4~18 min

80%~65% A;18~22 min

65%~62% A;22~25 min

62% A

25~30 min

62%~55% A;30~35 min

55% A

35～45 min

55%~50% A;45~50 min

50%~55% A;50~60 min

55%~80% A)

柱温30 ℃

流速0.8 mL·min-1

检测波长276

368 nm。结果: 甘草素、异甘草素、甘草查尔酮A的线性范围分别为0.050 4~0.403 2 (r=1)

0.039 4~0.315 2 (r=1)

1.638~8.192 μg (r=0.999 9);甘草素平均回收率为98.81%

RSD 1.7%;异甘草素平均回收率为99.16%

RSD 1.2%;甘草查尔酮A平均回收率为97.68%

RSD 1.0%。结论: 该方法简便、灵敏度高、专属性强

可用于甘蒲祛斑凝胶中3种指标性成分的含量测定。

Abstract

Objective: To establish a method for simultaneous determination of liquiritigenin

isoliquiritigenin and licochalcone A in Ganpuquban gel. Method: HPLC was applied on an Agilent HC-C18(4.6 mm×250 mm

5 μm) column by a gradient elution using (A) 0.05% phosphoric acid and (B) acetonitrile as the mobile phase(0-4 min

80%A;4-18 min

80%-65%A;18-22 min

65%-62%A;22-25 min

62% A

25-30 min

62%-55%A;30-35 min

55%A

35-45 min

55%-50%A;45-50 min

50%-55%A;50-60 min

55%-80%A). Detection wavelengths were set at 276

368 nm. The f low rate was 0.8 mL·min-1

the column temperature was maintained at 30 ℃. Result: The linear ranges of liquiritigenin

isoliquiritigenin and licochalcone A were 0.050 4-0.403 2 μg (r=1)

0.039 4-0.315 2 μg (r=1)

1.638-8.192 μg (r=0.999 9). The average recoveries were 98.81% with RSD 1.7%

99.16% with RSD 1.2% and 97.68% with RSD 1.0%

respectively. Conclusion: The established method is easy

accurate with high specificity

which can be used to determine the three components in Ganpuquban gel.

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