Objective: To investigate influence of temperature on quality of Dendrobii Officinalis Caulis tissue culture seedlings and provide a reference for artificial cultivation of this medicinal herb. Method: Dendrobii Officinalis Caulis tissue culture seedlings at multiplicative and rooting stage were setted in the environment of humidity

light intensity and illumination time controlled

setting temperature were 15

20

25

30

35 ℃

growth conditions of tissue culture seedlings were observed.Pearson correlation was analyzed

variance analysis was adopted to analyze growth index difference among each temperature. Result: Tissue culture seedlings's quality was good at 25-30 ℃ and they showed good growth and reproductive capacity.Pearson correlation analysis showed that temperature with seedling number and plant height were significantly correlated at 0.01 level in proliferation stage;temperature with plant height

leaf number and root number were significantly correlated at 0.01 level in rooting stage.LSD multiple comparison showed that difference of seedling number and plant height were obvious in proliferation stage

difference of leaf number

root number and plant height were obvious in rooting stage. Conclusion: Temperature is the key factor for quality of Dendrobii Officinalis Caulis tissue culture seedlings and the most suitable temperature is 25 ℃.