Objective: To investigate the biological basis of hypertension with hyperactivity of liver fire of macro characterization and behavioral tests

micro physicochemical indexes

disproof prescriptions. Method: Fifty-five SHR rats with hyperactivity of liver fire were divided into model group

Sancao Jiangya decoction (SCD) group

Sini San decoction (SND) group and nifedipine group according to Latin square method

Wistar-Kyoto (WKY) rats as control group. The macro characterization

behavioral indexes and the systolic blood pressure (SBP) were collected. Enzyme-linked immunosorbent assay(ELISA) was used to detect the serum concentration of 5-hydroxytryptamine(5-HT)

angiotensin Ⅱ (ANG Ⅱ)

norepinephrine (NE) after the rats were administrated for 4 and 6 weeks. Result: Compared with control group

the SBP and irritable degree increased

revolving tolerance time decreased

and serum 5-HT

ANGⅡ

NE level was higher in model group. Compared with model

the SBP in SCD group and nifedipine group reduced after treated for 4

6 weeks

(18

20 weeks of age)

the SBP in SND group was stable and increased in the 16 weeks

and then decreased. The symptoms of liver fire hyperactivity improved

and it was not obvious in SND group

and serum 5-HT

ANG Ⅱ

NE level was lower in SCD group compared with model group. Conclusion: Fourteen-eighteen weeks is the stable time window of hyperactivity of liver fire. SHR

5-HT

ANG Ⅱ

NE may be used as the objective index of hypertension of hyperactivity of liver fire syndrome.