Objective: To study the anti-gout effect and mechanism of Plantaginis Semen(PS) extract parts on the level of uric acid in hyperuricemia mice. Method: Five fractions including the petrol ether extract

the dichloromethane extract

the ethyl acetate extract

the butanol extract and the water-soluble extract were obtained by systematic solvent extraction. 90 Kunming mice were randomly divided into 9 groups:the normal group (normal saline)

the hyperuricemia group (normal saline)

the allopurinol group (0.01 g·kg-1)

the total extract of PS(TPS

25 g·kg-1) and the five fractions groups (25 g·kg-1).The hyperuricemia mice were induced by using oxygen oxazine acid potassium. The mice were administrated of the corresponding medicines for 7 days

and then the level of uric acid and the activity of xanthine oxidase were observed. Result: TPS and five fractions were obtained. Compared with the normal group

the level of uric acid and the activity of xanthine oxidase increased obviously in the hyperuricemia group (P<0.01). All treatment groups could reduce the level of serum uric acid obviously

and inhibit the activity of xanthine oxidase of hyperuricemia mice (P<0.05

P<0.01). Conclusion: The PS and its different extracts have good anti-gout effect in hyperuricemia mice.