Objective: To explore the chronic toxicity of Gansui Banxia Tang (GBT) on normal rat liver. Method: Eighty healthy SD rats were randomly divided into four groups:the normal group

the high-

middle-

and low-dose GBT groups (37

18.5

9.25 g·kg-1) of 20 in each group. The corresponding medicines at 0.02 mL·g-1were intragastrically administrated to rats for 90 days. After the last dose

blood samples of the half rats from each group were collected. The heart functions indexes including alanine aminotransferase (ALT)

aspartate aminotransferase (AST)

the ratio of AST/ALT

total bilirubin (TBIL)

direct bilirubin (DBIL)

and the blood lipid indexes including cholesterol (CHO)

triglyceride (TG)

high density lipoprotein (HDL)

low density lipoprotein (LDL) were detected. The morphology and histology of the liver were observed by HE staining. The remaining rats in each group were observed continuously for two weeks

and the above indexes were detected again. Result: In medicine taking

compared with the control group

the ALT

AST

AST/ALT

TBIL and DBIL had significant changes in high-dose GBT group (P<0.01)

the ALT

AST

DBIL (P<0.05) and AST/ALT(P<0.01) had significant changes in the middle-dose GBT group. The above indexes had no significant changes in the low-dose GBT group. The CHO

TG

HDL and LDL had no significant changes in all dose groups. The liver histopathological changes are gradually aggravated with the increase of the dose. While during the following observation

all the above indexes had no significant changes in all dose GBT groups. Conclusion: GBT has good effect on liver function and morphology of rats in a dose-dependent manner by long-term medication. Small dosage has little influence

the influence degree aggravates gradually as the increase of the dose

while the damage is reversible.