Objective: To explore the chronic toxicity of Gansui Banxia Tang (GBT) on the general situation

heart rate

cortisol level and adrenal gland morphology in normal rats. Method: Eighty healthy SD rats were randomly divided into four group:the normal group

the high-

middle-

and low-dose GBT groups (37

18.5 and 9.25 g·kg-1). The corresponding medicines at 0.02 mL·g-1were intragastrically administrated to rats for 90 days.The general situation changes including action

activity

diet

sleep

color

excrement and urine were observed once daily after administration. The body weight was observed twice weekly and the heart rate was recorded once before and once after the experiment. After the last dose

the blood samples were collected and the morphology and histology of the adrenal gland were observed by HE staining. Result: In medicine taking

no significant differences for the change of body weight

heart rate and cortisol were found between any two groups. Moreover

there were no obvious changes for the general situation changes including action

activity

diet

sleep

color

excrement

urine and adrenal gland morphology. Conclusion: GBT has no remarkable effects on the general situation

heart rate

cortisol level and adrenal gland morphology in normal rats by long-term medication.