Objective: To compare contents of five kinds of oligosaccharide esters in different processed products of Polygalae Radix and to discuss its variation before and after processing. Method: Contents of sibiricose A5

sibiricose A6

glomeratose A

tenuifoliside B and 3

6'-disinapoyl sucrose in different processed products of Polygalae Radix were determined by HPLC

mobile phase of acetonitrile-0.1% formic acid solution in gradient program and detection wavelength of 330 nm. Result: Contents of five oligosaccharide esters in root bark of Polygalae Radix were all obviously higher than them in the core.Contents of these five ingredients in root bark were 0.173%

0.155%

0.071%

0.120%

0.648%

contents of them in the core were 0.090%

0.053%

0.029%

0.018%

0.205%

respectively.In honey-stir-baked products

contents of sibiricose A6 and glomeratose A were higher than them in the crude

but sibiricose A5

tenuifoliside B and 3

6'-disinapoyl sucrose were lower than them in the crude.In licorice juice boiled products

contents of sibiricose A5

sibiricose A6

tenuifoliside B and 3

6'-disinapoyl sucrose were obviously lower than them in the crude

but glomeratose A was higher than them in the crude. Conclusion: Contents of five oligosaccharide esters are quite different between root bark and the core of Polygalae Radix

processing has a certain influence on contents of oligosaccharide esters.Sibiricose A5

sibiricose A6

tenuifoliside B and 3

6'-disinapoyl sucrose in Polygalae Radix all have qualiy change after boiled with licorice juice.