Objective: To optimize extraction technology of atractylenolide I

Ⅱ

Ⅲ from Atractylodis Macrocephalae Rhizoma. Method: HPLC was adopted to determine contents of atractylenolide I

Ⅱ

Ⅲ with mobile phase of acetonitrile (A)-water (B) for gradient elution (0-16 min

60%-76%A;16-18 min

76%-100%A;18-30 min

100%A) and dual-wavelength detection of 220 nm for atractylenolide I

Ⅱ and 276 nm for atractylenolide Ⅱ.Taking extracting rates of atractylenolide I

Ⅱ

Ⅲ as indexs

extraction method was screened by single factor test

orthogonal test was adopted to optimize extraction process with extracting time

extracting times

ethanol concentration and amount as factors. Result: Alcohol warm infusion method was selected

optimum technological conditions for extracting thrice by adding 6

4

4 times the amount of 90% ethanol at 40 ℃

extracting time was 1.5

1.0

1.0 h in turn.Extracting rates of atractylenolide I

Ⅱ

Ⅲ were 92.62%

92.39%

93.06%

respectively. Conclusion: This optimized extraction process is stable and feasible with high extraction efficiency of atractylenolide I

Ⅱ

Ⅲ in Atractylodis Macrocephalae Rhizoma.