Objective: To establish an UFLC-PDA method for the fingerprint of Pterocephali Herba

and compare the fingerprints of different medicinal parts. Method: The separation was performed on an Agilent Proshell 120 SB-C18 (4.6 mm×100 mm

2.7 μm) using gradient elution with water-formic acid (100:0.2) and acetonitrile

at a flow rate of 1.0 mL·min-1. The column temperature was kept at 30 ℃

and detection wavelength was set at 238 nm. Result: The UFLC-PDA fingerprints of 17 batches were set up. The results showed that 15 chromatographic peaks were extracted as the common peaks of fingerprint of aboveground part

while in underground part peak 6 and peak 7 were nonexistent. 5 peaks were identified as chlorogenic acid

loganin

sweroside

evodia rutaecarpa glycosides and triplostoside A. The similarity degrees were above 0.9 excepted 6 batches of aboveground part. Conclusion: The method has good presicion

stability and repeatability that it could provide basis for quality control and evaluation of Pterocephali Herba