多波长HPLC同时测定痛安注射液中4种有效成分

林夏; 崔培超; 仲艳; 胡军华; 李家春; 黄文哲; 王振中; 萧伟

doi:10.13422/j.cnki.syfjx.2015090050

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多波长HPLC同时测定痛安注射液中4种有效成分

化学与分析 | 更新时间：2024-01-04

- 多波长HPLC同时测定痛安注射液中4种有效成分
- Simultaneous Determination of Four Active Components in Tong'an Injection by HPLC Under Multiple UV Wavelengths
- 中国实验方剂学杂志 2015年21卷第9期页码：50-53
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家“重大新药创制”科技重大专项(2013ZX09402203)
- DOI：10.13422/j.cnki.syfjx.2015090050
  中图分类号： O657.72;R286.0
- 纸质出版日期：2015
- 稿件说明：
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林夏, 崔培超, 仲艳, 等. 多波长HPLC同时测定痛安注射液中4种有效成分[J]. 中国实验方剂学杂志, 2015,21(9):50-53.

LIN Xia, CUI Pei-chao, ZHONG Yan, et al. Simultaneous Determination of Four Active Components in Tong'an Injection by HPLC Under Multiple UV Wavelengths[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(9): 50-53.
林夏, 崔培超, 仲艳, 等. 多波长HPLC同时测定痛安注射液中4种有效成分[J]. 中国实验方剂学杂志, 2015,21(9):50-53. DOI： 10.13422/j.cnki.syfjx.2015090050.

LIN Xia, CUI Pei-chao, ZHONG Yan, et al. Simultaneous Determination of Four Active Components in Tong'an Injection by HPLC Under Multiple UV Wavelengths[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(9): 50-53. DOI： 10.13422/j.cnki.syfjx.2015090050.

摘要

目的: 建立同时测定痛安注射液中木兰花碱、原阿片碱、黄连碱、白屈菜碱含量的HPLC方法. 方法: 采用Gemini C18色谱柱(4.6 mm×250 mm

5 μm)

流动相乙腈(A)-0.02 mol·L-1磷酸二氢钾(B)梯度洗脱

流速1.0 mL·min-1

检测波长230 nm (木兰花碱)

290 nm(原阿片碱、白屈菜碱)

360 nm(黄连碱)

柱温30 ℃. 结果: 木兰花碱、原阿片碱、黄连碱、白屈菜碱分别在10.856～108.560 (r=0.999 8)

24.712～247.120 (r=0.999 6)

3.464～34.640 (r=0.999 6)

39.296～392.960 mg·L-1 (r=0.999 9)线性关系良好

回收率在97.5%~101.8%. 结论: 建立的方法准确可靠

可以用于测定痛安注射液中木兰花碱、原阿片碱、黄连碱、白屈菜碱的含量.

Abstract

Objective: To establish an HPLC method for content determination of magnoflorine

protopine

coptisine and chelidonine in Tong'an injection. Method: Gemini C18(4.6 mm×250 mm

5 μm)was used with the mobile phase of acetonitrile-0.02 mol·L-1 monopotassium phosphate at a flow rate of 1.0 mL·min-1. The detection wavelength was set at 230 nm for magnoflorine

290 nm for protopine and chelidonine

360 nm for coptisine

and the column temperature was maintained at 30 ℃. Result: Calibration curves of the magnoflorine

protopine

chelidonine and coptisine in Tong'an injection showed good linear relationship:10.856-108.560(r=0.999 8)

24.712-247.120(r=0.999 6)

3.464-34.640(r=0.999 6)

39.296-392.960 mg·L-1 (r=0.999 9). The average recoveries of the components above were between 97.5%-101.8%. Conclusion: The method was feasible

accurate and stable

which could be used for determination of magnoflorine

protopine

coptisine and chelidonine in Tong'an injection.

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