Objective: To establish a method for determination of HPLC fingerprint of Stigma Maydis

provide reference

and basis for a quality standard of Stigma Maydis pieces. Method: Analysis was performed on an Agilent Zorbax SB-C18 (4.6 mm×250 mm

5 μm)

with the methanol-0.2% phosphoric acid as mobile phase. The gradient elution was carried out. The flow rate was 0.8 mL·min-1. The column temperature was 30 ℃. The injection volume was 10 μL. Result: HPLC fingerprint was established based on 24 batches of Stigma Maydiswere 18 common peaks

and two of them were identified asrutin(peak 11)

apigenin(peak 15). Conclusion: Cluster analysis and principal component analysis should also participate in quality evaluation of Chinese herbal medicine

the HPLC fingerprin provides a reference for the quality standards for Stigma Maydis.