Objective: To investigate the chemical constituents in the extract of Zhike Chuanbei Pipa dropping pills. Method: The compounds were isolated from the extract of Zhike Chuanbei Pipa dropping pills by a combination of various chromatographic techniques including column chromatography over silica gel

reversed-phase silica gel

sephadex LH-20 columns

and preparative HPLC. Their structures were identified by spectroscopic analysis including MS and NMR data. Result: Twenty-four compoundswere isolated and identified as nerolidol-3-O-{α-L-rhamnopyranosyl-(l→4)-α-L-rhamnopyranosyl-(l→2)-[α-L-rhamnopyranosyl-(l→6)]-β-D-glucopyranoside} (1)

nerolidol-3-O-α-L-rhamnopyranosyl-(l→4)-α-L-rhamnopyranosyl-(l→6)-β-D-glucopyranoside (2)

nerolidol-3-O-{α-L-rhamnopyranosyl-(l→4)-α-L-rhamnopyranosyl-(l→2)-[4-trans-feruloyl-α-L-rhamnopyranosyl-(l→6)]-β-D-glucopyranoside} (3)

(6S

7E

9R)-6

9-dihydroxy-4

7-megastigmadien-3-one 9-O-β-D-apiofuranosyl-(l→6)-β-D-glucopyranoside (4)

4-caffeoylquinic acid (5)

chlorogenic acid (6)

(6S

7E

9R)-9-hydroxy-4

7-megastigmadien-3-one 9-O-β-D-glucopyranoside (7)

eriobotrosideⅡ(8)

3

4-dihydroxy-benzoic acid (9)

(6R

7E

9R)-9-hydroxy-4

7-megastigmadien-3-one 9-O-β-D-glucopyranoside (10)

(+) lyoniresinol 9'-O-β-D-xylopyranoside (11)

(-) lyoniresinol 9'-O-β-D-xylopyranoside (12)

fumaric acid (13)

2

6-dimethoxy-4-(2-propenyl)phenol 1-O-β-D-glucopyranoside (14)

(+)lyoniresinol (15)

kaempferol (16)

4-hydroxy phenol (17)

4-hydroxy benzoic acid (18)

4-hydroxy cinnamic acid (19)

(6S

7E

9R)-6

9-dihydroxy-4

7-megastigmadien-3-one (20)

euscaphic acid (21)

ursolic acid (22)

3-furancarboxylic acid (23)

vanillic acid (24). Conclusion: Among the isolated twenty-four compounds

there are eight sesquiterpenoids (1

2

3

4

7

8

10

20)

four phenylpropanoids (11

12

14

15)

nine organic acids (5

6

9

13

17

18

19

23

24)

two triterpenoids (21

22)

one flavonoid (16). These compounds were obtained from the extract of Zhike Chuanbei Pipa dropping pills for the first time.