Objective: In this study

we investigated the impact of combining Kansui Radix with Glycyrrhizae Radix et Rhizoma(1: 4) on the kidney in rats. On this basis

the mechanism of the reducing toxicity was also studied. Method: Male Sprague-Dawley (SD) rats were randomly assign to four groups

which were controls

Kansui Radix

Glycyrrhizae Radix et Rhizoma and Kansui Radix-Glycyrrhizae Radix et Rhizoma(1: 4).The kidney extract metabonomic analyses of Kansui Radix

Glycyrrhizae Radix et Rhizoma and Kansui Radix-Glycyrrhizae Radix et Rhizoma(1: 4) was used by NMR and OPLS-DA. Clinical biochemical and histopathological assessments were also performed. Result: All the clinical biochemical

histopathological and metabolites results were no significant change in Glycyrrhizae Radix et Rhizoma treated rats. Not only the increased glucose and decreased creatine/creatinine in plasma

but also the renal tubular epithelial cell swelling and vacuoles degeneration

glomerular blood clot were observed in Kansui Radix treated rats. Besides

decreased choline

phosphorylcholine

phenylalanine and glutamine

increased isoleucine

leucine

alanine

valine

acetic acid

creatine/creatinine was observed in Kansui Radix treated rats. In Kansui Radix-Glycyrrhizae Radix et Rhizoma(1: 4)treatment group

there was no significant change in plasma index. The histopathological changes was only the glomerular congestion and renal tubular epithelial cells expansion. The variations were only declined of choline

phosphorylcholine and phenylalanine in Kansui Radix. Conclusion: Detoxification of Kansui Radix in kidney by combining with Glycyrrhizae Radix et Rhizoma was obvious. It could be owing to the intervention of the biomarker of Kansui Radix and down regulation of the traditional indicator of kidney lesion.