Objective: The aim of this study was to investigate the metabolites and pathways of salvianolic acid B(SAB) in rats. Method: In this study

12 SD-rats were divided into 2 groups

with 6 rats used for collecting the blank plasma

bile

urine excretion samples

the others used for collecting the samples after intravenous administration of SAB(100 mg·kg-1). UPLC-Q-TOF method was used to implement the qualitative analysis and identification of structure of the metabolites in each sample after treatmentwere. Result: According to the mass information

11 metabolites were discovered

with 2 metabolites including SAB ester bond hydrolysis product in plasma

5 metabolites including danshensu

lithospermic acid (LSA)

the decarboxylation and methylation metabolites of LSA in bile

4 metabolites including SAB methylation and sulfated products in urine excretion. Conclusion: SAB could metabolize into various metabolites including danshensu. The metabolic reaction of SAB occurs mainly in the ester bond and five-member ring

via ester bond hydrolysis

molecular dehydration

decarboxylation

hydroxylation

methylation and sulfate

etc.