北青龙衣总多糖脱蛋白工艺比较

任晓蕾; 霍金海; 董文婷; 张海燕; 王伟明

doi:10.13422/j.cnki.syfjx.2015100016

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北青龙衣总多糖脱蛋白工艺比较

药剂与炮制 | 更新时间：2024-01-04

- 北青龙衣总多糖脱蛋白工艺比较
- Comparison of Deproteinization Process for Total Polysaccharides from Exocarp of Unripe Fruits of
- 中国实验方剂学杂志 2015年21卷第10期页码：16-18
- 作者机构：
- 作者简介：
- 基金信息：
  
  哈尔滨市科技局青年科技创新人才项目(2013RFQYJ056);黑龙江省科技攻关项目(GC12C110);国家“重大新药创制”科技重大专项(2009ZX09102-138)
- DOI：10.13422/j.cnki.syfjx.2015100016
  中图分类号： R283.6
- 纸质出版日期：2015
- 稿件说明：
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任晓蕾, 霍金海, 董文婷, 等. 北青龙衣总多糖脱蛋白工艺比较[J]. 中国实验方剂学杂志, 2015,21(10):16-18.

REN Xiao-lei, HUO Jin-hai, DONG Wen-ting, et al. Comparison of Deproteinization Process for Total Polysaccharides from Exocarp of Unripe Fruits of [J]. Chinese journal of experimental traditional medical formulae, 2015, 21(10): 16-18.
任晓蕾, 霍金海, 董文婷, 等. 北青龙衣总多糖脱蛋白工艺比较[J]. 中国实验方剂学杂志, 2015,21(10):16-18. DOI： 10.13422/j.cnki.syfjx.2015100016.

REN Xiao-lei, HUO Jin-hai, DONG Wen-ting, et al. Comparison of Deproteinization Process for Total Polysaccharides from Exocarp of Unripe Fruits of [J]. Chinese journal of experimental traditional medical formulae, 2015, 21(10): 16-18. DOI： 10.13422/j.cnki.syfjx.2015100016.

摘要

目的: 优选北青龙衣总多糖的脱蛋白工艺. 方法: 以蛋白质脱除率和总多糖保留率为指标

通过单因素试验和正交试验比较Sevage法、三氯乙酸法(TCA)、木瓜蛋白酶法、酶-Sevage联用法及酶-TCA联用法对北青龙衣总多糖的脱蛋白质效果. 结果: 木瓜蛋白酶-TCA法除蛋白效果最好

最佳脱蛋白条件为木瓜蛋白酶用量3%

酶解时间2 h

酶解温度60 ℃

pH 6

TCA质量分数6%.蛋白质脱除率84.76%

总多糖保留率82.48%. 结论: 不同脱蛋白质法对北青龙衣总多糖中蛋白质脱除效果有明显差异

酶-TCA法效果最佳且操作简便、耗时短.

Abstract

Objective: To optimize deproteinization process of total polysaccharides from exocarp of unripe fruits of Juglans mandshurica. Method: With retention rate of total polysaccharides and deproteinized rate as indexes

effects of Sevage

trichloroacetic acid(TCA)

papain

enzyme-Sevage and enzyme-TCA on deproteinization of total polysaccharides were compared and optimized by single factor tests and orthogonal test. Result: Effect of papain-TCA method to take off protein was the best

optimum conditions were as follows:papain dosage of 3%

hydrolysis temperature of 60 ℃

hydrolysis time of 2 h

pH of 6

the concentration of TCA 6%.Retention rate of total polysaccharides and deproteinized rate were 82.48% and 84.76%

respectively. Conclusion: Deproteinized effects on total polysaccharides from exocarp of unripe fruits of J. mandshurica have significant difference by different deproteinized methods

enzyme-TCA combination method is easy to operate with optimum deproteinized effect and short time-consuming.

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