Objective: To optimize extraction process of Paozhenkang spray. Method: Taking composite score of the content of berberine hydrochloride and dry extract rate as index

orthogonal test was adopted to investigate influence of extraction time

extraction times

the amount of water on water extraction process.With composite score of the total content of aloe-emodin

rhein

emodin

chrysophanol

physcion and dry extract rate as index

orthogonal test was adopted to optimize alcohol extraction process by taking ethanol concentration

alcohol amount

extraction time and times as factors.Contents of berberine hydrochloride and five aglycones from Rhei Radix et Rhizoma were determined by HPLC. Result: Optimun water extraction process consisted of adding 12-fold of waters

decocting 3 times

90 min for each time

the content of berberine hydrochloride was 6.07%.Optimun alcohol extraction process was as following:refluxing extracted twice with 10-fold of 75% ethanol

75 min for each time;the total content of five aglycones was 1.96%. Conclusion: These optimized extraction processes are stable and feasible for industrial production of Paozhenkang spray.