Objective: To study the chemical constituents and coagulation Factor Xa (FXa) inhibition activities of Semen Persicae. Method: Chromogenic-substrates and continuous rate methods were used to assess the FXa inhibition activities. Various chromatographic techniques were used to isolate and purify the major active compounds

and their structures were identified by spectral analysis. Result: The ethyl acetate part possessed inhibition of 60.27% to FXa

and ten compounds were isolated and identified as 9-octadecenoic acid-2

3-dihydroxypropyl ester (1)

daucosterol (2)

2-hydroxy-2-phenylacetamide (3)

benzoic acid (4)

2-hydroxy-9-octadecenoic acid (5)

n-heptadecanoic acid (6)

methyl elaidate (7)

p-hydroxybenzoic acid (8)

palmitic acid (9) and amygdalin (10). Conclusion: The ethyl acetate part was the major FXa inhibition active site. Compounds 1 and 3 were isolated from the family of Rosaceae for the first time

and compounds 5-8 were first reported in this plant.