JI Bao-yu, PEI Li-xin, CHEN Sui-qing, et al. Total Genomic DNA Extraction from Dried and Fresh Herbs of Corni Fructus[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(10): 70-72.
JI Bao-yu, PEI Li-xin, CHEN Sui-qing, et al. Total Genomic DNA Extraction from Dried and Fresh Herbs of Corni Fructus[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(10): 70-72. DOI: 10.13422/j.cnki.syfjx.2015100070.
Objective: To explore the best total genomic DNA extraction method from the dried and fresh Corni Fructus and to compare the DNA quality as a foundation for future research. Method: The new modified CTAB method
SDS method were used to get DNA
the content of nucleic acid and agarose gel electrophoresis were tested. Result: The OD260/OD280 ratios of DNA extracted by using the new modified CTAB method A were between 1.7-1.9 and the agarose gel electrophoresis results showed that the bands of the DNA were both tight and distinct. Compared with the DNA samples from dried herbs
results showed that DNA quality from fresh herbs was better. Conclusion: New modified CTAB is a simple and fast method for extract the DNA in high quantity and quality from herbs
the obtained DNA by this method is suitable for further studies of molecular biology.