HPLC同时测定利胆片中绿原酸和黄芩苷的含量

孟姝; 林玉莲; 都述虎

doi:10.13422/j.cnki.syfjx.2015100077

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HPLC同时测定利胆片中绿原酸和黄芩苷的含量

化学与分析 | 更新时间：2024-01-04

- HPLC同时测定利胆片中绿原酸和黄芩苷的含量
- Simultaneous Determination of Baicalin and Chlorogenic Acid in Lidan Tablets by HPLC
- 中国实验方剂学杂志 2015年21卷第10期页码：77-79
- 作者机构：
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- DOI：10.13422/j.cnki.syfjx.2015100077
  中图分类号： O657.72;R286.0
- 纸质出版日期：2015
- 稿件说明：
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孟姝, 林玉莲, 都述虎. HPLC同时测定利胆片中绿原酸和黄芩苷的含量[J]. 中国实验方剂学杂志, 2015,21(10):77-79.

MENG Shu, LIN Yu-lian, DU Shu-hu. Simultaneous Determination of Baicalin and Chlorogenic Acid in Lidan Tablets by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(10): 77-79.
孟姝, 林玉莲, 都述虎. HPLC同时测定利胆片中绿原酸和黄芩苷的含量[J]. 中国实验方剂学杂志, 2015,21(10):77-79. DOI： 10.13422/j.cnki.syfjx.2015100077.

MENG Shu, LIN Yu-lian, DU Shu-hu. Simultaneous Determination of Baicalin and Chlorogenic Acid in Lidan Tablets by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(10): 77-79. DOI： 10.13422/j.cnki.syfjx.2015100077.

摘要

目的: 建立了高效液相色谱法同时测定利胆片中黄芩苷和绿原酸含量的方法. 方法: 采用Capcell Pak C18色谱柱(4.6 mm×150 mm

5 μm)

流动相乙腈(A)-0.3%磷酸水(B)梯度洗脱(0~8 min

87%A;8~9 min

87%~67%A;9~15 min

67%A;15~16 min

67%~87%A;16~20 min

87%A)

检测波长315 nm

柱温30 ℃

流速1 mL ·min-1

进样量10 μL. 结果: 利胆片中黄芩苷和绿原酸两种成分均达到基线分离

黄芩苷在0.001 0~0.049 9 g ·L-1(r=0.999 9)线性关系良好

平均回收率98.62% (RSD 1.1%);绿原酸在0.005 3~0.026 7 g ·L-1(r=0.999 3)线性关系良好

平均回收率98.98% (RSD 0.3%). 结论: 该测定方法快速

结果准确、可靠

可用于利胆片中黄芩苷和绿原酸的含量测定.

Abstract

Objective: The contents of baicalin and chlorogenic acid in Lidan tablets were simultaneously determined by HPLC. Method: The sample were separated on Capcell Pak C18 (4.6 mm×150 mm

5 μm) column

which eluted with acetonitrile (A) and 0.3% phosphoric acid (B). The ratio of acetonitrile and water increased from 87:13 to 67:33 in 20 min with detected wavelength at 315 nm

flow rate at 1 mL · min-1

column temperature at 30 ℃ and injection volume was 10 μL. Result: The result showed that two compounds reach the baseline separation

the linear range of baicalin and chlorogenic acid was 0.001 0-0.049 9 g · L-1 (r=0.999 9) and 0.005 3-0.026 7 g · L-1 (r=0.999 3)

respectively

and the average recovery of baicalin and chlorogenic acid was 98.62% (RSD 1.1%) and 98.98% (RSD 0.3%)

respectively. Conclusion: The method is rapid

precise and practical that it can be used to determine the two components in Lidan tablets.

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