Objective: To set up an content determination method for oleanolic acid and ursolic acid in Lycopi Herba by HPLC

and to find the relationship between altitude and medicinal material content. Method: AgilentTC C18 column(4.6 mm×250 mm

5 μm) was selected

the mobile phase was methanol-acetonitrile-0.2% glacial acetic acid solution(78:10:12)

the flow rate was 0.8 mL · min-1. The column temperature performed at 40 ℃

and the target component was detected at 210 nm. Result: Oleanolic acid showed a linear ranges at 0.164-2.46 μg(r=0.999 8) and ursolic acid was 0.325 6-4.884 μg(r=0.999 8). The average recovery of the added sample were 99.03% (RSD 1.7%) and 98.83% (RSD 1.4%)

respectively. Altitude and herbal content showed a certain degree of positive correlation. Conclusion: The method is simple and rapid

accurate and reliable

therefore available for the elevation of Lycopi Herba. Relationship between altitude and quality can be a preferably guidance for planting area.