Objective: The aim of the study was to investigate effects and mechanism of Danhong Huayu Koufuye (DHK) on diabetic retinopathy and diabetic nephropathy based on the aspect of thrombus formation. Method: Effects of DHK on the clotting time and bleeding time were observed by capillary glass tube method and transecting the tip of the tail

respectively. Antithrombotic effects of DHK were examined with the ligation model of inferior vena cava

carotid artery-carotid vein bypass and ferric chloride-induced arterial thrombosis in rats. The animals in the study of the clotting time

bleeding time

the ligation model of inferior vena cava and carotid artery-carotid vein bypass model were randomly divided into 4 groups:model group

low dosage of DHK(0.60

2.25

1.60

1.60 g · kg-1 · d-1)

high dosage of DHK(1.20

4.50

3.20

3.20 g · kg-1 · d-1)

aspirin group(2.88

11.26

7.81

7.81 mg · kg-1 · d-1)

respectively. Rats in ferric chloride-induced arterial thrombosis experiments were divided into 5 groups:sham group

model group

low and high dosage of DHK(1.60

3.20 g · kg-1 ·d-1)

and clopidogrel group(30 mg · kg-1 · d-1). Clotting time was assayed after single and multiple administration for 5 days. Bleeding time and the thrombus weight were detected after administered for 5 days. Result: Compared with vehicle control

DHK significantly prolonged the clotting time in New Zealand rabbits (P<0.05) and bleeding time in mice (P<0.05). High dosage of DHK significantly inhibited thrombus formation. Inhibition rate of thrombus dry weight in high dosage DHK group was 73.8% in ligation model of inferior vena cava

and 45.1% in carotid artery-the carotid vein bypass model. Thrombus percentage of high dosage DHK group was (52.1±17.7)% (P<0.05; vs model group) in ferric chloride-induced arterial thrombosis in rats. Conclusion: DHK has activities of promoting blood circulation to remove blood stasis and prevent thrombus formation.