Objective: To investigate effect of “treating winter disease in summer” prescriptionon compatibility on percutaneous absorption of tetrahydropalmatine. Method: Franz-type diffusion cell was employed

taking isolated rat skin as transdermal barrier

HPLC was adopted to determine the content of tetrahydropalmatine in in vitro percutaneous absorption liquid with mobile phase of methanol-0.1% formic acid

detection wavelength at 280 nm and flow rate of 0.8 mL·min-1.Cumulative permeation quantities and penetration rates of tetrahydropalmatine in “treating winter disease in summer” patches with different prescription compatibility were calculated. Result: In vitro percutaneous behavior of tetrahydropalmatine complied with the zero-order kinetics equation.Penetration rate (1.04 μg·cm-2·h-1) and cumulative permeation quantity (29.54 μg·cm-2) of the whole group were greater than a single herb and difference was statistically significant.By comparing with the lack of Kansui Radix group and the lack of Asari Radix et Rhizoma group

effect of the whole group on in vitro percutaneous absorption of tetrahydropalmatine had no significant

however

compared with the lack of Semen Sinapis

difference was statistically significant. Conclusion: “Treating winter disease in summer” prescription compatibility has a certain influence on percutaneous absorption of tetrahydropalmatine

Semen Sinapis can enhance transdermal absorption of tetrahydropalmatine.