Box-Behnken设计-效应面法优化荆防止痒颗粒中挥发油提取工艺

郭宜城; 谭志江; 丁志军; 肖丽丽; 罗美兰

doi:10.13422/j.cnki.syfjx.2015110032

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Box-Behnken设计-效应面法优化荆防止痒颗粒中挥发油提取工艺

药剂与炮制 | 更新时间：2024-01-04

- Box-Behnken设计-效应面法优化荆防止痒颗粒中挥发油提取工艺
- Optimization of Extraction Process of Volatile Oil from Jingfang Zhiyang Granules by Box-Behnken Design Response Surface Methodology
- 中国实验方剂学杂志 2015年21卷第11期页码：32-35
- 作者机构：
- 作者简介：
- 基金信息：
  
  江西省科技计划项目(赣科发计字[2007]189号)
- DOI：10.13422/j.cnki.syfjx.2015110032
  中图分类号： O657.72;R286.0
- 纸质出版日期：2015
- 稿件说明：
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郭宜城, 谭志江, 丁志军, 等. Box-Behnken设计-效应面法优化荆防止痒颗粒中挥发油提取工艺[J]. 中国实验方剂学杂志, 2015,21(11):32-35.

GUO Yi-cheng, TAN Zhi-jiang, DING Zhi-jun, et al. Optimization of Extraction Process of Volatile Oil from Jingfang Zhiyang Granules by Box-Behnken Design Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(11): 32-35.
郭宜城, 谭志江, 丁志军, 等. Box-Behnken设计-效应面法优化荆防止痒颗粒中挥发油提取工艺[J]. 中国实验方剂学杂志, 2015,21(11):32-35. DOI： 10.13422/j.cnki.syfjx.2015110032.

GUO Yi-cheng, TAN Zhi-jiang, DING Zhi-jun, et al. Optimization of Extraction Process of Volatile Oil from Jingfang Zhiyang Granules by Box-Behnken Design Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(11): 32-35. DOI： 10.13422/j.cnki.syfjx.2015110032.

摘要

目的: 优化荆防止痒颗粒中挥发油的提取工艺

为该制剂的临床应用提供参考. 方法: 采用HPLC测定胡薄荷酮的含量

流动相甲醇-水(80 :20)

检测波长252 nm.以挥发油提取量和挥发油中胡薄荷酮含量为指标

采用Box-Behnken设计-效应面法考察浸泡时间、提取时间及液料比对荆防止痒颗粒中挥发油提取工艺的影响. 结果: 最佳提取工艺为浸泡时间2 h

提取时间6.5 h

料液比9 mL·g-1.挥发油提取量0.69 mL

胡薄荷酮质量浓度38.17 g·L-1

与预测值的偏差较小. 结论: 优选的提取工艺合理可行

可有效保留荆防止痒颗粒中挥发性成分.

Abstract

Objective: To optimize extraction process of volatile oil from Jingfang Zhiyang granules. Method: HPLC was employed to determine the content of pulegone with mobile phase of methanol-water and detection wavelength at 252 nm.Taking yield of volatile oil and the content of pulegone as indexes

Box-Behnken design-response surface methodology was adopted to optimize extraction process of volatile oil with soaking time

extraction time and solid-to-liquid ratio as factors. Result: Optimum extraction process was as follows:soaked 2 h and extracted 6.5 h with 9 times the amount of water.Yield of volatile oil was 0.69 mL with the content of pulegone was 38.17 g·L-1

these values were similar to their predictive values. Conclusion: This optimized extraction process is reasonable and feasible

which can effectively retain volatile ingredients in Jingfang Zhiyang granules.

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