黄精多糖水解物柱前衍生HPLC指纹图谱

秦垂新; 曹子丰; 黄绮敏; 林伟斌

doi:10.13422/j.cnki.syfjx.2015110065

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黄精多糖水解物柱前衍生HPLC指纹图谱

化学与分析 | 更新时间：2024-01-04

- 黄精多糖水解物柱前衍生HPLC指纹图谱
- Pre-column Derivatives HPLC Fingerprint of Polygonati Rhizoma Polysaccharide Hydrolysate
- 中国实验方剂学杂志 2015年21卷第11期页码：65-68
- 作者机构：
- 作者简介：
- 基金信息：
  
  广东省部产学研结合项目(2012B090600025)
- DOI：10.13422/j.cnki.syfjx.2015110065
  中图分类号： R284.1
- 纸质出版日期：2015
- 稿件说明：
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秦垂新, 曹子丰, 黄绮敏, 等. 黄精多糖水解物柱前衍生HPLC指纹图谱[J]. 中国实验方剂学杂志, 2015,21(11):65-68.

QIN Chui-xin, CAO Zi-feng, HUANG Qi-min, et al. Pre-column Derivatives HPLC Fingerprint of Polygonati Rhizoma Polysaccharide Hydrolysate[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(11): 65-68.
秦垂新, 曹子丰, 黄绮敏, 等. 黄精多糖水解物柱前衍生HPLC指纹图谱[J]. 中国实验方剂学杂志, 2015,21(11):65-68. DOI： 10.13422/j.cnki.syfjx.2015110065.

QIN Chui-xin, CAO Zi-feng, HUANG Qi-min, et al. Pre-column Derivatives HPLC Fingerprint of Polygonati Rhizoma Polysaccharide Hydrolysate[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(11): 65-68. DOI： 10.13422/j.cnki.syfjx.2015110065.

摘要

目的: 建立黄精多糖柱前衍生HPLC指纹图谱. 方法: 以三氟乙酸(TFA)水解黄精多糖

水解产物加入1-苯基-3-甲基-5-吡唑啉酮(PMP)进行衍生化

采用HPLC测定单糖的衍生物.采用ZORBAX Eclipse XDB-C18色谱柱(4.6 mm×250 mm

5 μm)

流动相0.1 mol·L-1磷酸缓冲液(pH 6.7)-乙腈(84.5 :15.5)

检测波长250 nm

柱温30 ℃

流速0.8 mL·min-1. 结果: 黄精多糖柱前衍生指纹图谱标示出10个共有峰

鉴别了7个共有峰(D-甘露糖

D-半乳糖醛酸

L-鼠李糖

D-半乳糖

D-葡萄糖

D-木糖

D-阿拉伯糖).10批黄精药材指纹图谱相似度 >0.99. 结论: 该方法简便

分离度高

重复性及稳定性良好

可有效控制黄精多糖的质量.

Abstract

Objective: To establish a pre-column derivation HPLC method of chromatographic fingerprint of Polygonati Rhizoma polysaccharide. Method: Polygonati Rhizoma polysaccharide was hydrolyzed with trifluoroacetic acid and derivated by 1-phenyl-3-methyl-5-pyrazolone (PMP). The monosaccharide composition was separated by reversed-phase technique on a Zorbax Eclipse XDB-C18 column (4.6 mm×250 mm

5 μm) with a mobile phase composed of 0.1 mol·L-1phosphate buffer (pH 6.7) and acetonitrile in the ratio of 84.5 : 15.5.The flow rate was 0.8 mL·min-1. The detection wavelength was set at 250 nm

and column temperature was at 30 ℃. Result: There were 10 common peaks from the fingerprint and 7 peaks were identified as D-mannose

L-rhamnose

D-galacturonic

D-galactose

D-glucose

D-xylose

and D-arabinose. The similarity of 10 batches of Polygonati Rhizoma polysaccharide was more than 0.99. Conclusion: The method is accurate and reproducible

which can be used for the quality control of Polygonati Rhizoma.

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