Objective: To study the effect of ShanJing capsule on nuclear transcription factor-κB (NF-κB) signal pathway relating apoptosis proteins caspases-3 (Caspase-3)

anti-apoptotic genes B cell lymphoma/leukemia-2(Bcl-2)

pro-apoptotic genes Bcl-2 associated X protein(Bax)

explore its protective effects on myocardial ischemia and mechanisms. Method: Wistar rats the coronary artery of which were ligated were used for myocardial ischemia model

the changes of the ST section in electrocardiogram(ECG) were observed

and randomly divided into 5 groups:model (0.9% sodium chloride)

Shanjing capsule high

medium and low dose (respectively 283.5

850.5

1 701.0 mg·kg-1)

Metoprolol capsules(324.0 mg·kg-1)

compared with sham group (0.9% sodium chloride). All groups were administered once daily for 10 days. The ST segment

serum lactate dehydrogenase (LDH)

creatine kinase (CK)

aspartate aminotransferase (AST) levels were observed. Immunohistochemistry was used to detect the NF-κB

Caspase-3

Bax

Bcl-2 protein of myocardial tissue. Result: Compared with the sham group

ST segment elevation was significantly raised (P <0.05)

LDH

CK and AST activity was significantly higher (P <0.05)

NF-κB

Caspase-3

Bax's protein expression levels of myocardial tissue were significantly higher (P <0.05)

Bcl-2 protein expression was significantly lower (P <0.05) in the model group;Shanjing capsule could obviously ameliorate the raise of ST segment

and lower activity of CK

LDH and AST. Shanjing capsule could obviously decrease the protein expressions of NF-κB

Caspase-3 and Bax and increase the protein expression of Bcl-2. Conclusion: Shanjing capsule could regulate the expressions of NF-κB signaling pathway relating proteins

inhibit the myocardial apoptosis and reduce ischemia anoxic pathological damage of myocardial muscle cells to protect the myocardial muscle.