Objective: In order to evaluate the inducing toxicity effect of Aconiti Lateralis Radix Praeparata and different compatibility proportion of Ginseng Radix et Rhizoma in neonatal rats cardiomyocytes. Method: The primary culture cardiac cells (6 d) were randomly divided into normal(NC) group

Aconiti Lateralis Radix Praeparata and Ginsang Radix et Rhizoma(AG) group

blood serum(BS) group. The inducing toxicity effect of blood serum of different compatibility proportion of Aconiti Lateralis Radix Praeparata and Ginseng Radix et Rhizoma on spontaneous imp frequency

cell viability

superoxde dismutase(SOD)

malondialdehyde(MDA)

Nitric oxide (NO) and lactate dehydrogenase(LDH) was investigated in vitro in neonatal rat cardiomyocytes. The activity of Caspases-3

the expression of Bcl-2 and Bax protein

the expression of Bcl-2 and Caspases-3 mRNA was assayed by spectrophotometry

western blot and real-time PCR respectively. Result: Compared with AG1:0 group

the content of SOD increased obviously

spontaneous imp frequency

the content of MDA

the secretory volume of NO and the release rate of LDH decreased obviously

the activity of Caspases-3

the expression of Bax protein and Caspases-3 mRNA decreased obviously

the expression of Bcl-2 protein and Bcl-2 mRNA increased gradually

cell viability increased in 2-4 h significantly (P <0.05) in other AG groups. Compared with BS group

there was no significant difference in AG1 : 0.5 group in the above indexes. Conclusion: There was a significant inducing toxicity effect of Aconiti Lateralis Radix Praeparata and different compatibility proportion of Ginseng Radix et Rhizoma in neonatal rats cardiomyocytes

the optimization compatibility proportion in AG1:0.5.