多指标综合评分法优选敷胸巴布贴醇提工艺

韩毅; 于艳; 王雪峰; 吴振起; 马明悦; 周旭

doi:10.13422/j.cnki.syfjx.2015120005

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多指标综合评分法优选敷胸巴布贴醇提工艺

药剂与炮制 | 更新时间：2024-01-04

- 多指标综合评分法优选敷胸巴布贴醇提工艺
- Optimization of Ethanol Extraction Process for Fuxiong Cataplasms by Multi-index Comprehensive Scoring Method
- 中国实验方剂学杂志 2015年21卷第12期页码：5-8
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目(81273800)
- DOI：10.13422/j.cnki.syfjx.2015120005
  中图分类号： R284.2
- 纸质出版日期：2015
- 稿件说明：
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韩毅, 于艳, 王雪峰, 等. 多指标综合评分法优选敷胸巴布贴醇提工艺[J]. 中国实验方剂学杂志, 2015,21(12):5-8.

HAN Yi, YU Yan, WANG Xue-feng, et al. Optimization of Ethanol Extraction Process for Fuxiong Cataplasms by Multi-index Comprehensive Scoring Method[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(12): 5-8.
韩毅, 于艳, 王雪峰, 等. 多指标综合评分法优选敷胸巴布贴醇提工艺[J]. 中国实验方剂学杂志, 2015,21(12):5-8. DOI： 10.13422/j.cnki.syfjx.2015120005.

HAN Yi, YU Yan, WANG Xue-feng, et al. Optimization of Ethanol Extraction Process for Fuxiong Cataplasms by Multi-index Comprehensive Scoring Method[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(12): 5-8. DOI： 10.13422/j.cnki.syfjx.2015120005.

摘要

目的: 优选敷胸巴布贴的醇提工艺。方法: 以大黄素、黄芩苷、桑辛素含量及浸膏得率的综合评分为指标

利用正交试验考察乙醇体积分数、溶剂用量、提取时间及提取次数对敷胸巴布贴醇提工艺的影响。采用HPLC测定大黄素及黄芩苷含量

流动相分别为甲醇-0.4%磷酸溶液(80:20)

甲醇-0.4%磷酸溶液(47:53)

检测波长分别为254

280 nm。采用紫外分光光度法测定桑辛素含量。结果: 最佳醇提工艺为加6倍量75%乙醇提取2次

每次3 h。大黄素、黄芩苷、桑辛素质量分数及浸膏得率分别为0.19%

3.61%

0.51%

31.19%。结论: 优选的提取工艺合理可行

有效成分提取效率高。

Abstract

Objective: To optimize ethanol extraction process of Fuxiong cataplasms. Method: With dry extract yield and contents of emodin

baicalin

morusin as indexes

orthogonal design was carried out for optimizing extraction process by taking the concentration and amount of ethanol

extracting time and times as factors

optimum process was determined by taking composite score for condition.Contents of emodin and baicalin were determined by HPLC

mobile phases were consisted of methanol-0.4% phosphoric acid (80: 20) and (47: 53)

detection wavelengths were 254 nm and 280 nm

respectively.The content of morusin was determined by UV spectrophotometry. Result: Optimum extraction technology was as follows:extracted twice with 6 times the amount of 75% ethanol for 3 h of each time.Dry extract yield and contents of emodin

baicalin

morusin were 31.19%

0.19%

3.61% and 0.51%

respectively. Conclusion: This optimized extraction technology is rational with high extraction rates of active components.

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