Objective: To extract high quality genomic DNA from dry leaves of Artemisia rupestris dried naturally and lie a foundation for further molecular experiments. Method: Three different methods

conventional hexadecyl trimethyl ammonium bromide (CTAB) method and modified CTAB methods were applied to isolate genomic DNA from dry leaves of A. rupestris.Two modified CTAB methods were different from conventional CTAB method in three ways

nucleus lysate(STE) was used for removal of impurities

polyvinyl pyrrolidone (PVP) was added when grinding samples and 95% ethanol for precipition of DNA in room temperature.These two modified CTAB methods were different from whether liquid nitrogen was added while grinding samples.Purity and integrality of DNA were detected by UV spectrophotometry

agarose gel electrophoresis and sequence-related amplified polymorphism (SRAP). Result: Higher purity DNA was obtained by improved CTAB methods by comapred with conventional CTAB method

value of A260 nm/A280 nmwas between 1.8-2.0

polysaccharides was removed relatively clear.SRAP produced clear polymorphic patterns. Conclusion: These two modified CTAB methods are suitable for DNA extraction from dry leaves of A. rupestris

purity and integrality of isolated DNA can be used in further molecular biology research.