Objective: To study active constitunents of secondary metabolites in endophytic fungus from Cephalotaxus fortunei root. Method: Antibacterial and antitumor activity of ethyl acetate extracts from fermentation liquor of an endophytic fungus (CEP18) in C. fortunei root were tested by filter paper diffusion method and MTT

active ingredients were analyzed by GC-MS. Result: Secondary metabolites in endophytic fungus from C. fortunei root significantly inhibited growth of Bacillus subtilis

Staphylococcus aureus and Human promyelocytic leukemia cells (HL-60).After silica gel column chromatography analysis and activity test

five components (Fr1-Fr5) were isolated from ethyl acetate extracts

the Fr2 was identified as active component

which was mainly composed of butyl lactate (2.96%)

2-chloro ketone warfarin (8.65%)

(Z)-11-eicosenamide (13.90%)

1-methyl pyrene (3.51%)

dimethyl succinate (6.34%)

4-hydroxyphenylacetic acid (8.27%)

elaidic acid-methyl ester (10.07%)

linoleic acid (5.60%) and hexadecanoic acid (7.54%). Conclusion: 4-Hydroxyphenylacetic acid is reported for the first time in C. genus at present research and is worthy of further development and utilization.