Objective: To investigate effect of compatibility of Paeoniae Radix Alba and Aconiti Radix Cocta on transdermal permeation role of paeoniflorin. Method: Modified Franz diffusion cell was adopted with excised mice skin as transdermal barrier

through in vitro percutaneous absorption test

the content of paeoniflorin was determined by HPLC

then cumulative amount

permeation rate and lag time were calculated in order to obtain penetration curve equation. Result: Steady-state permeation rates of paeoniflorin according to groups of Paeoniae Radix Alba

Paeoniae Radix Alba-Aconiti Radix Cocta

Paeoniae Radix Alba+1% azone

Paeoniae Radix Alba+1% oleic acid and Paeoniae Radix Alba+1% propylene glycol were 0.818

2.532

3.974

0.621

0.430 μg·cm-2·h-1;apparent permeability coefficients of them were 2.538×10-8

7.856×10-8

12.328×10-8

1.928×10-8

1.335×10-8 cm·s-1;lag times were 6.168

0.071

10.462

1.487

11.827 h

respectively. Conclusion: Compatibility of Paeoniae Radix Alba and Aconiti Radix Cocta is benefit to transdermal permeation of paeoniflorin.