Objective: To optimize purification technology of total flavonoids from Hyssopus officinalis by macroporous resin. Method: Adsorption and desorption capacity of total flavonoids was taken as index

11 different types of macroporous resins were screened

static adsorption-elution test were carried out.Single factor test was used to investigate purification technology of total flavonoids from H. officinalis. Result: AB-8 macroporous resin was the most suitable one for purification of total flavonoids in H. officinalis.Optimum purification parameters were as followings:the sample concentration of 2.56 g·L-1

sample flow rate of 4 BV·h-1

diameter-high ratio of resin bed 1: 5

washed impurity with 5 BV of water

elution flow rate of 5 BV·h-1 and discarded eluent

eluted with 9 BV of 70% ethanol at 4 BV·h-1.Purity of total flavonoids reached 65.6%. Conclusion:This purification technology is stable

feasible and suitable for purification of total flavonoids from H. officinalis.