三黄泻心汤水、醇提取物抗炎作用比较及有效提取物的成分分析

姚佳琪; 肖永庆; 刘颖; 于定荣; 麻印莲; 李鹏远; 李丽

doi:10.13422/j.cnki.syfjx.2015130031

您当前的位置：

首页 >

文章列表页 >

三黄泻心汤水、醇提取物抗炎作用比较及有效提取物的成分分析

化学与分析 | 更新时间：2024-01-04

- 三黄泻心汤水、醇提取物抗炎作用比较及有效提取物的成分分析
- Anti-inflammatory Effect Comparison of Water and Ethanol Extracts of Sanhuang Xiexin Tang and Component Analysis of Its Active Extracts
- 中国实验方剂学杂志 2015年21卷第13期页码：31-35
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金面上项目(81274087)
- DOI：10.13422/j.cnki.syfjx.2015130031
  中图分类号： R285.5
- 纸质出版日期：2015
- 稿件说明：
移动端阅览
姚佳琪, 肖永庆, 刘颖, 等. 三黄泻心汤水、醇提取物抗炎作用比较及有效提取物的成分分析[J]. 中国实验方剂学杂志, 2015,21(13):31-35.

YAO Jia-qi, XIAO Yong-qing, LIU Ying, et al. Anti-inflammatory Effect Comparison of Water and Ethanol Extracts of Sanhuang Xiexin Tang and Component Analysis of Its Active Extracts[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(13): 31-35.
姚佳琪, 肖永庆, 刘颖, 等. 三黄泻心汤水、醇提取物抗炎作用比较及有效提取物的成分分析[J]. 中国实验方剂学杂志, 2015,21(13):31-35. DOI： 10.13422/j.cnki.syfjx.2015130031.

YAO Jia-qi, XIAO Yong-qing, LIU Ying, et al. Anti-inflammatory Effect Comparison of Water and Ethanol Extracts of Sanhuang Xiexin Tang and Component Analysis of Its Active Extracts[J]. Chinese journal of experimental traditional medical formulae, 2015, 21(13): 31-35. DOI： 10.13422/j.cnki.syfjx.2015130031.

摘要

目的: 通过比较三黄泻心汤水、醇提取物的抗炎作用

确定有效提取物并对有效提取物进行成分分析

探讨三黄泻心汤抗炎的药效物质基础。方法: 以二甲苯所致的小鼠耳肿胀为抗炎活性筛选模型

口服灌胃给药

通过计算各给药组的小鼠耳肿胀抑制率

确定三黄泻心汤的有效提取物

并运用HPLC法对有效提取物进行化学成分分析。结果: 与模型组比较

各给药组肿胀度均有所下降

醇提物的大、中剂量组能够显著降低小鼠耳肿胀度(P<0.01)

肿胀抑制率约46%;2种提取物相同剂量组间比较

醇提物的大、中剂量组肿胀度显著低于水提物(P<0.05)

醇提物的抗炎活性优于水提物。黄酮类-生物碱类-蒽醌类成分在醇提物中的质量比约9:2:5。结论: 三黄泻心汤醇提物具有显著的抗炎作用

是该方抗炎的有效提取物

其抗炎作用与有效提取物中化学成分的组成及各类成分的量比关系密切相关。

Abstract

Objective: To determine the active extracts and uncover the material basis of Sanhuang Xiexin Tang

comparing the anti-inflammatory effect of its water and ethanol extracts. Method: Use the xylene-induced auricular swelling of mouse as screening model: oral administration

and calculate the inhibition degree of swelling to determine the active extracts. Meanwhile

conduct the component analysis for active extracts. Result: Compared with model group

decrease of swelling in different groups is observed

the swelling of mouse is significantly lower of ethanol extracts than water extracts (P<0.01) and the inhibition degree is about 46%. The swelling of mouse in the medium dose and high dose of ethanol extracts are significantly lower than water extracts (P<0.05)

the inflammatory effect of ethanol extract is better than water extracts. The ratio of flavone

anthraquinone and alkaloid in ethanol extracts is 9: 2: 5. Conclusion: The inflammatory effect of ethanol extracts is obvious

which has something to do with the component ratio within the extracts. And ethanol extracts is determined as the active extracts of Sanhuang Xiexin Tang.

关键词

Keywords

references

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

色空间法结合化学计量学判别当归炭的炮制程度

中药多糖改善心肌缺血再灌注损伤的作用机制

沙棘多糖提取工艺、结构特征及其药理活性研究进展

中药黄酮类化合物改善脑缺血再灌注损伤的作用及其机制研究进展

基于质量和药理效应比较姜半夏一体化饮片与传统饮片