Objective: To identificate different origins and categories of commodity edible bird's nest (EBN) by DNA barcoding technique fastly and acurrately. Method: Total genomic DNA was isolated from different origins and categories of commodity EBN. Nicotinamide adenine dinucleotide dehydrogenase subunit 2 (ND2) gene sequences were amplified and sequenced.Identification were performed using similar Blast from GenBank

nearest distance and neighbor-joining (NJ) methods. Result: NJ phylogenetic tree showed 31 white or silver EBN samples which were clustered with Aerodramus fuciphagus;a black EBN sample was clustered with A. maximus.However

among white or silver EBN samples

there were 8 samples clustered into a separate branch with support rate of 82%

which were identified by cytochrome b (Cytb) gene as the subspecies (A. fuciphagus germani)

and sequence alignment showed 2 parsimony-informative sites.This result showed that segment of ND2 sequence can identify subspecies of A. fuciphagus. Conclusion: ND2 sequence is efficient barcode for identifying origin species of EBN.