Objective: To establish a HPLC method for the simultaneous determination of three main flavonoids in Sabina chinensis and Junipeius formosana. Method: Samples were extracted by 70 % methanol with ultrasonic process. The HPLC was performed on an CAPCELL PAK C18 column (4.6 mm×250 mm

5 μm)with 0.2%H3PO4acetonitrile-0.2%H3PO4 water as mobile phase. The flow rate was 1.0 mL·min-1 and the column temperature was kept at 40 ℃. The detection wavelength was at 360 nm. Result: The linear range of rutin was 0.004 9-0.146 4 μg(r=0.999 9);the linear rang of quercetin-3-rhamnoside was 0.009 4-0.281 4 μg(r=0.999 8)and the linear range of kaempferol was 0.005 4-0.162 0 μg(r=0.999 8). The average recoveries of rutin

quercitrin;and kaempferol were 100.12%(RSD 2.6%)

96.35%(RSD 0.7%)

96.13%(RSD 0.5%)

respectively. Conclusion: Three flavonoids reached baseline separation within 40 min. This method has advantages of less analytic time

good stability and accuracy of operation

and has certain reference value for quality control of Junipeius formosana and Sabina chinensis herb.